Immobilization of urease enzyme on chitosan/polyvinyl alcohol electrospun nanofibers.

Electrospun nanofibers have gained much attention for enzyme immobilization due to their high surface-to-volume ratio. In this study, urease was immobilized on chitosan/poly(vinyl alcohol) (PVA) nanofibers by both adsorption and crosslinking methods. In order to obtain nanofibers with more desirable properties, solutions with different ratios of chitosan and PVA were electrospun and crosslinked using glutaraldehyde. Comparing SEM images of the nanofibers, before and after immersing them in phosphate buffer, it was shown that higher chitosan content leads to more stable fibers. So, the solution with the chitosan to PVA ratio of 40:60 was used for enzyme immobilization. Then, the effects of initial protein concentration, temperature, incubation time, and method of immobilization were investigated to reach the highest enzyme activity. Under similar immobilization conditions, covalently immobilized urease showed higher activity, compared to uncrosslinked immobilized enzyme. Besides, it retained 30% of its initial activity after 10 times usage. So, this method was chosen for further investigation. Not only the activity of the immobilized enzyme was much higher than the free enzyme in a wide range of pH and temperature, but also stability of the immobilized enzyme was improved. Immobilized urease was then used to remove thiourea which is a toxic compound. Findings indicated 60% hydrolysis of initial thiourea in 12 h. In conclusion, the findings showed that chitosan/PVA nanofibers are suitable candidates for the immobilization of urease.

Inhibition performances of graphene oxide/silver nanostructure for the microbial corrosion: molecular dynamic simulation study.

Steel is one of the mainly used materials in the oil and gas industry. However, it is susceptible to the marine corrosion, which 20% of the total marine corrosion is caused by microbiologically influenced corrosion (MIC). The economic and environmental impacts of corrosion are significant, and it is crucial to fight against corrosion in a proper sustainability context and environmental-friendly methods. In this study, the graphene oxide/silver nanostructure (GO-Ag) inhibitory effect on the corrosion of steel in the presence of sulfate reducing bacteria (SRB) was investigated, via weight loss (WL) and Tafel polarization measurements. Moreover, molecular dynamic (MD) simulations were performed to obtain a deep understanding of the corrosion inhibition effect of GO-Ag. GO-Ag showed a significant antibacterial effect at 80 ppm. Moreover, WL and Tafel polarization measurements illustrated a great inhibition efficiency, which reached up to 84% reduction of WL and 98% reduction of corrosion current density (Icorr) after 7 days of incubation with GO-Ag. Based on MD simulations, bonding energy reached to the larger value in the presence of GO-Ag, which indicated the ability of graphene oxide nanosheets to be adsorbed on the steel surface and prevent the access of corrosive agents to the steel surface. The radial distribution function (RDF) results implied distance between corrosive agent (water and SRB) and steel surface (Fe atoms), which indicated protection of the steel surface due to the effective adsorption of GO nanosheets through the active sites of the steel surface. The mean square displacement (MSD) result showed smaller displacement of the corrosive particles on the surface of steel, resulting that the GO-Ag molecules bonded with Fe molecules on the surface of steel.

Green mitigation of microbial corrosion by copper nanoparticles doped carbon quantum dots nanohybrid.

Recently, nanomaterials have been introduced as a new generation of inhibitors to control the microbiologically influenced corrosion (MIC). In this study, copper nanoparticles doped carbon quantum dots (Cu/CQDs) nanohybrid was used as an inhibitor to reduce the MIC. FESEM, EDS, FTIR, and XRD were used to characterize the nanohybrid. The dose-response test was performed to evaluate the inhibitory effect of Cu/CQDs against SRB. Design-Expert software was used to design the matrix of experiment and analyze the result. Cu/CQDs showed significant inhibitory effect against SRB compared to the copper nanoparticles (CuNPs) and carbon quantum dots (CQDs), at 50 ppm. Moreover, corrosion behavior of X60 steel was evaluated via electrochemical impedance spectroscopy (EIS) and Tafel polarization techniques in the presence of SRB and Cu/CQDs. The fitted result of EIS showed that the charge transfer resistance (Rct) value increased in the presence of Cu/CQDs owing to the enhancement in the thickness of the electrical double layer, indicating that Cu/CQDs is able to provide significant corrosion protection to X60 steel in the presence of SRB. In addition, FESEM, EDS, and XRD were used to study the formed corrosion products and biofilm on the surface of X60 steel. Corrosion test results indicated that the addition of the Cu/CQDs reduced the surface damage of X60 steel in the presence of SRB. It is attributed to the carbon dots adsorption film formation, which possessed a significant protective ability to inhibit the corrosion of steel in the presence of SRB.